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  • NEUROSYIL Phenyl Preparative Columns: 300Å

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    • NEUROSYIL Phenyl Preparative Columns: 300Å

    NEUROSYIL Phenyl Preparative Columns: 300Å

    NEUROSYIL Phenyl preparative  300A column is a type of HPLC (High-Performance Liquid Chromatography) column used for preparative chromatography, typically for protein, peptide, and other biomolecule purification. The term "Phenyl 300A" refers to:

    1. Phenyl Stationary Phase :

    • The stationary phase is phenyl-functionalized (a phenyl or benzene ring attached to the silica surface).
    • Phenyl groups provide π–π interactions and hydrophobic interactions, useful for separating aromatic compounds and biomolecules with aromatic amino acids.
    • Offers different selectivity compared to C18 or C8 phases.

    2. 300 Å (Angstrom) Pore Size

    • The 300A pore size (equivalent to 30 nm) is large, designed for large molecules like:
      • Proteins
      • Peptides
      • Oligonucleotides
    • Smaller pore sizes (e.g., 100Å) are better for small molecules, but they restrict diffusion of large biomolecules.

    3. Preparative Scale

    • Preparative columns are larger in diameter (typically 10–50 mm ID or more) and are designed for purification and collection of milligram to gram quantities of target compounds.

    They are often used downstream after analytical-scale method development

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