National Chromatography Inco
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Ultimasyil preparative diol 100A is a type of HPLC column manufactured by NCIN (Nci Scientific Pvt. Ltd.), using high-purity silica and featuring a diol stationary phase with a 100 Angstrom (Å) pore size, designed for normal phase and size exclusion chromatography applications. This column is suitable for separating biomolecules like proteins and peptides, as well as separating steroids and sterols under normal phase conditions, and can also perform gel filtration with an aqueous buffer.
Key Features & Specifications
How it Works
The diol functional groups in the Ultimasyil 100A column act as a polar phase, providing unique selectivity for compounds with high hydrophilicity.
In Aqueous Buffers:
The diol groups create a hydrophilic surface, which shields the underlying silica from interacting with basic proteins, allowing for their separation via gel filtration.
In Normal Phase:
The column interacts with non-polar compounds, separating them based on their varying polarity.
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